CONSTRUCTION OF A GENETIC MAP FOR ARABICA COFFEE USING AFLP MARKERS

Authors: PEARL, HEATHER - HI AG RES CNT; NAGAI, CHIFUMI - HI AG RES CNT; Moore, Paul; STEIGER, DENISE - HI AG RES CNT; OSGOOD, ROBERT - HI AG RES CNT; MING, RAY - HI AG RES CNT

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/18/2002
Publication Date: 1/11/2003
Citation: Pearl, H.M., Nagai, C., Moore, P.H., Steiger, D.L., Osgood, R.V., Ming, R. 2003. Construction of a genetic map for arabica coffee using AFLP markers. (Abs.) Plant, Animal & Microbe Genome XI: P551, pg 212. 2003.

Interpretive Summary: Abstract only.

Technical Abstract: Molecular marker linkage maps are being developed as scaffolds for phenotype mapping in many crop plants to assist directed germplasm improvement through marker assisted technologies. We are using amplified fragment length polymorphisms (AFLPs) to construct a genetic linkage map on a pseudo F2 population of arabica coffee (Coffea arabica L.) derived from a cross between the cultivars Mokka Hybrid and Catimor. The Mokka Hybrid cultivar, commercially grown in Hawaii, produces high quality beverage, has small bean size and small, narrow leaves. The Catimor cultivar, derived from a hybrid between C. arabica and C. canephora backcrossed to C. arabica, does not produce high quality coffee and has large bean size and resistance to coffee rust, Hemileia vastatrix Berk. Sixty-two trees from this population were selected for construction of a linkage map. A total of 321 dominant markers and eight co-dominant markers were generated from 150 AFLP primer combinations. Segregation distortion was observed on 163 of the 329 markers. Additional markers are being added to this working linkage map for more complete coverage of the arabica coffee genome. This genetic map will be used for mapping QTLs controlling coffee quality and productivity.