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United States Department of Agriculture

Agricultural Research Service

Title: Quantification of Mycosphaerella Graminicola in Wheat by Real-Time Pcr

Authors
item Adhikari, Tika
item Balaji, Boovaraghan - PURDUE UNIVERSITY
item Breeden, Jill
item Anderson, Joseph
item Goodwin, Stephen

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: July 31, 2004
Publication Date: July 31, 2004
Citation: Adhikari, T., Balaji, B., Breeden, J.D., Anderson, J.M., Goodwin, S.B. 2004. Quantification of Mycosphaerella graminicola in wheat with real-time PCR. Phytopathology. 94:S2.

Technical Abstract: Septoria tritici blotch caused by Mycosphaerella graminicola is a serious fungal disease of wheat worldwide. Developing wheat cultivars resistant to M. graminicola is complicated by a long latent period, environmental effects on symptom expression, and variation among evaluators in the use of disease scoring systems. To circumvent these problems, we developed a fluorescent real-time PCR assay to quantify the biomass of M. graminicola in wheat cultivars. A set of specific primers was designed from B-tubulin gene sequences, which amplified a PCR product of 100 bp from pure cultures and infected wheat leaves only. The real-time PCR analysis showed that the biomass of M. graminicola and disease severity in the resistant cultivars, Tadinia and W7984, were significantly less than those in either of the susceptible checks, Yecoro Rojo and Opata85, beginning 12 d after inoculation. Real-time PCR appears to be a valuable tool for discriminating between resistant and susceptible wheat.

Last Modified: 12/28/2014
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