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ARS Home » Pacific West Area » Davis, California » Western Human Nutrition Research Center » Immunity and Disease Prevention Research » Research » Publications at this Location » Publication #161223
Title: GLUCOSE AND CATIONIC AMINO ACID TRANSPORTER EXPRESSION IN GROWING CHICKENS (GALLUS GALLUS DOMESTICUS)

Author
item HUMPHREY, BROOKE - UNIV OF CALIF DAVIS
item Stephensen, Charles
item CALVERT, CHRIS - UNIV OF CALIF DAVIS
item KLASING, KIRK - UNIV OF CALIF DAVIS

Submitted to: Comparative Biochemistry and Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/23/2004
Publication Date: 6/16/2004
Citation: Humphrey, B.D., Stephensen, C.B., Calvert, C.C., Klasing, K.C. 2004. GLUCOSE AND CATIONIC AMINO ACID TRANSPORTER EXPRESSION IN GROWING CHICKENS (GALLUS GALLUS DOMESTICUS). Comparative Biochemistry and Physiology. 138:515-525.

Interpretive Summary: Tissue glucose transporter (GLUT1-3) and cationic amino acid transporter (CAT1-3) mRNA expression was determined in growing broiler chicks posthatch. In two experiments, tissues were either collected on d 1, 3 and 7 or d 1 and 14 posthatch. Heart and liver were the only tissues expressing a GLUT isoform on d 1. All tissues expressed a GLUT isoform on d 7 except for the thymus. Most tissues expressing a CAT isoform on d 1 decreased mRNA levels through d 7 (P<0.05), except for bursa CAT-1 which tended to increase (P=0.05). The thymus and spleen did not express any CAT isoform mRNA until d 7. The liver was the only tissues expressing GLUT-2 mRNA through d 14. On d 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues (P<0.05). High affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively (P<0.05). Total CAT mRNA expression was greatest in the bursa P<0.05). The thymus had the lowest high affinity GLUT and total CAT mRNA expression on d 14 posthatch. Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.

Technical Abstract: Tissue glucose transporter (GLUT1-3) and cationic amino acid transporter (CAT1-3) mRNA expression was determined in growing broiler chicks posthatch. In two experiments, tissues were either collected on d 1, 3 and 7 or d 1 and 14 posthatch. Heart and liver were the only tissues expressing a GLUT isoform on d 1. All tissues expressed a GLUT isoform on d 7 except for the thymus. Most tissues expressing a CAT isoform on d 1 decreased mRNA levels through d 7 (P<0.05), except for bursa CAT-1 which tended to increase (P=0.05). The thymus and spleen did not express any CAT isoform mRNA until d 7. The liver was the only tissues expressing GLUT-2 mRNA through d 14. On d 14, GLUT-1, CAT-1 and CAT-2 mRNA were differentially expressed across tissues (P<0.05). High affinity GLUT and CAT mRNA expression was highest in the heart and bursa, respectively P<0.05). Total CAT mRNA expression was greatest in the bursa (P<0.05). The thymus had the lowest high affinity GLUT and total CAT mRNA expression on d 14 posthatch. Therefore, T lymphocytes within the thymus may be most susceptible to glucose and cationic amino acid supply.