ARS | Publication request: Development and Characterization of Fiber Est-Derived Microsatellites in Cotton (Gossypium Spp).

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Proceedings/Symposium
Publication Acceptance Date: January 6, 2004
Publication Date: January 6, 2004
Citation: PARK, Y.H., ULLOA, M., SICKER, B.A., WILKINS, T. 2004. DEVELOPMENT AND CHARACTERIZATION OF FIBER EST-DERIVED MICROSATELLITES IN COTTON (GOSSYPIUM SPP). NATIONAL COTTON COUNCIL BELTWIDE COTTON CONFERENCE. p. 1187-1191.

Technical Abstract: A new set of microsatellite or simple sequence repeat (SSR) markers were developed from the sequence information of a fiber EST database from the diploid species Gossypium arboreum. Four hundred EST-derived SSR primer sets were designed and tested for PCR amplification. Polymorphic loci among six species of cotton were observed from 233 (73.7 %) SSR markers and 83 were monomorphic. Between G. hirsutum and G. barbadense, 103 markers were polymorphic, while the polymorphism within these two tetraploid species was low 4.7 % and 3.8 %, respectively. One hundred twenty-three SSR markers that were polymorphic among the species showed sequence similarity to the genes with known function including fiber elongation-related genes. Based on PCR amplification, the transferability of EST-derived SSR marker from the diploid species G. arboreum was high (89.9 %) across the six Gossypium species. Most of these SSR markers provide information about functional gene, demonstrated portability and strength, and may be useful for diverse genome analysis and for the improvement of the cotton crop through marker-assisted selection.