Submitted to: Carotenoid Gordon Conference
Publication Type: Abstract Only
Publication Acceptance Date: October 8, 2003
Publication Date: January 4, 2004
Citation: Miller, H.B. 2004. The measurement of carotenoids in stackburn rice. Carotenoid Gordon Conference, Ventura, CA, Jan. 4-9, 2004. Technical Abstract: Yellow rice that occurs during poor storage conditions is called stackburn. Stackburn rice is discarded by commercial rice mills but may contain carotenoids. We developed a novel use of synchronous fluorescence spectrometry to non-destructively identify the presence of carotenoids in a single kernel. Our technique takes advantage of two characteristics of the yellowed rice kernel: the high absorptivity of carotenoids and the high autofluorescence of rice endosperm. We have confirmed that the discarded yellow kernels contain carotenoids. We have also developed a small (test tube) scale system for investigating the induction of carotenoids during stackburn. Every rice cultivar tested underwent stackburn to some extent. The induction of carotenoid synthesis requires a high temperature of around 70oC and requires a fairly specific water content. And, while the literature indicates a fungal component to stackburn, we have eliminated this possibility in our system.