Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2003
Publication Date: December 1, 2003
Citation: Rodriguez, M.F., Hansen, J., Wiens, G.D., Welch, T.J., Palti, Y. 2003. Characterization and mapping of immune response genes in rainbow trout (oncorhynchus mykiss). Meeting Abstract. Nov 9-15, 2003. p.4. Technical Abstract: Diseases constitute a main constraint to productivity in aquaculture, yet very little is known about fish immune systems. To implement effective health management practices in aquaculture, a better understanding of the genetic basis of disease resistance in fish is required. In our current research we sequence, characterize, and map immune response genes in rainbow trout to obtain the necessary information for future QTL (quantitative trait loci) mapping and functional genomic analyses. The major histocompatibility (MH) class I and II genes play a major role in pathogen immunity, and they have been genetically and physically mapped in rainbow trout. We are sequencing the entire MH class I and II regions to develop genetic markers and characterize MH haplotypes in our breeding stocks. We use cDNA probes to screen a BAC (bacterial artificial chromosome) library and to identify clones that contain immune response loci. The positive BACs are sequenced to identify and develop microsatellite and SNP (single nucleotide polymorphism) markers, and for characterization of promoters and other regulatory elements. The markers are integrated into the NCCCWA rainbow trout genetic linkage map and will be used for identifying QTL that affect immune response and disease resistance.