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United States Department of Agriculture

Agricultural Research Service


item Stern, Norman
item Svetoch, Edward - ST RES CTR RUSSIA
item Eruslanov, Boris - ST RES CTR RUSSIA
item Kovalev, Yuri - ST RES CTR RUSSIA
item Volodina, Larisa - ST RES CTR RUSSIA
item Perelygin, Vladimir - ST RES CTR RUSSIA
item Mitsevich, Evgeni - ST RES CTR RUSSIA
item Mitsevich, Irina - ST RES CTR RUSSIA
item Pokhilenko, Victor - ST RES CTR RUSSIA
item Borzenkov, Valery - ST RES CTR RUSSIA

Research conducted cooperatively with:
item The Russian State Research Centre For Applied Microbiology

Submitted to: International Association for Food Protection
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2004
Publication Date: August 11, 2004
Citation: Stern, N.J., Svetoch, E.A., Eruslanov, B.V., Kovalev, Y.N., Volodina, L.I., Perelygin, V.V., Mitsevich, E.V., Mitsevich, I.P., Pokhilenko, V.D., Borzenkov, V.N. 2004. Development of a novel therapeutic treatment of chickens to control Campylobacter jejuni colonization [Abstract]. International Association for Food Protection. p. 114.

Technical Abstract: Campylobacter jejuni is an important foodborne agent of human gastroenteritis. This organism is thought to be transmitted most frequently through exposure to poultry products. We screened 365 Bacillus/Paenibacillus spp. isolates from poultry production to identify potentials for anti-C. jejuni activity. Zones of C. jejuni inhibition surrounding 56 isolates piqued our interest. One novel antagonistic Bacillus circulans (NRRL B-30644) and two Paenibacillus polymyxa (NRRL B-30507 & NRRL B-30509) strains were identified and deposited under provisions of the Budapest Treaty. The cell-free, ammonium sulfate precipitate from each candidate culture also created zone of C. jejuni inhibition in spot tests. Exposure of the crude antimicrobial preparation to protease enzymes ablated Campylobacter inhibition, thus demonstrating a peptide characteristic consistent with bacteriocin definition. The pure peptides were obtained by gel-filteration, ion-change chromotography and hydrophobic interaction. The peptides were characterized by SDS-PAGE electrophoresis; isoelectric focusing; and amino acid sequencing. In 15 separate experiments, one or two days-post-hatch chicks were colonized with challenges of ~2 x 10(6) cfu C. jejuni, and placed in isolation units. Three days before sampling, therapeutic feeds were provided ad-libitum. This feed consisted of purified bacteriocin (0.25 or 0.5 g) micro-encapsulated in polyvinyl-pyrrolidone and incorporated into 1 Kg of chicken feed. Therapeutic treatment consistently reduced C. jejuni colonization by at least 100,000 fold over the untreated chicks. Therapeutic bacteriocin treatment of mature chickens prior to slaughter may substantially reduce public exposure to this organism.

Last Modified: 7/30/2016
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