Title: MICROCHIP CAPILLARY ELECTROPHORESIS-BASED GENETIC COMPARISON OF CLOSELY RELATED CYATHOSTOMIN NEMATODE PARASITES OF HORSES USING RANDIMLY AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION.
Posedi, Janez - U LJUBLJANA, SLOVENIA
Drogemuller, Michaela - INST PARASITOLOGY,GERMAN
Schneider, Thomas - INST PARASITOLOGY,GERMANY
Hoglund, Johan - SWEPAR, SWEDEN
Samson-Himmelstjerna, Georg Von - INST PARSITOLOGY,GERMANY
Submitted to: Parasitology Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 5, 2003
Publication Date: January 12, 2004
Citation: Posedi, J., Drogemuller, M., Schneider, T., Hoglund, J., Lichtenfels, J.R., Samson-Himmelstjerna, G. 2004. Microchip capillary electrophoresis-based genetic comparison of closely related cyathostomin nematode parasites of horses using randimly amplified polymorphic dna polymerase chain reaction.Parasitology Research. 92:421-429.
Nematodes of the subfamily Cyathostominae are now regarded as the principal parasitic pathogen of the horse. The 40 species in horses worldwide can be identified by a few authorities using comparative anatomy of adult stages, but larval stages are exceptionally difficult to identify and eggs are impossible to identify to even subfamily level. The development of DNA- based methods that can be used to identify all stages of the nematodes including eggs in the feces is a high priority need. This report describes the use of microchip-based capillary electrophoresis technology for the analysis of complex DNA banding patterns to identify the closely related cyathostomin species Cylicocyclus elongatus and C. insigne from the horse. The results will be used by researchers in the pharmaceutical industry, the Food and Drug Administration, and other scientists developing more efficient diagnostic methods for evaluating biological control agents and antiparasitic drugs for controlling these important nematode parasites of horses worldwide.