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United States Department of Agriculture

Agricultural Research Service

Title: Transformation of Barley with Two Antifungal Genes

Authors
item Dahleen, Lynn
item Manoharan, M. - UOFA, PINE BLUFF, AR

Submitted to: National Fusarium Head Blight Forum Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 18, 2003
Publication Date: December 13, 2003
Citation: Dahleen, L.S., Manoharan, M. 2003. Transformation of barley with two antifungal genes. National Fusarium Head Blight Forum Proceedings. p 12.

Technical Abstract: Insertion and expression of multiple antifungal genes has the potential of increasing resistance to a variety of fungal diseases. Using particle bombardment and bialaphos selection for the bar gene, multiple barley plants containing both a rice thaumatin-like protein (tlp) gene and a rice chitinase (chi) gene were regenerated from three transformation events. Southern analysis confirmed integration of the transgenes into the barley genome. Northern analysis of T0 plants indicated that event 1 did not contain RNA from either gene. Plants from event 2 expressed both genes at high levels, while plants from event 3 showed small amounts of tlp RNA but no chi RNA. Western analysis of T1 progeny confirmed that event 2 lines expressed both genes and event 3 lines expressed only tlp. Homozygous T2 lines have been identified and are being examined for gene expression levels for both transgenes.

Last Modified: 10/1/2014
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