|Ma, Xeufeng - UNIV OF MISSOURI|
Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: November 2, 2003
Publication Date: November 20, 2003
Citation: Ma, X.F., Gustafson, J.P. 2003. Rye genome variation in wheat backgrounds [abstract]. Crop Science Society Of America. p. C-7261245. Technical Abstract: Rye genome variation in wheat backgrounds was studied at a genome-wide level by using AFLP and RFLP analyses to investigate the genome banding profiles of four primary triticales, and two sets (14) of wheat/rye addition lines. The results indicated that wheat parental AFLP bands were highly conserved, whereas rye bands underwent great changes in triticales and 90 percent of those changes were also detected in the corresponding wheat/rye addition lines. The AFLP bands amplified by methylation sensitive primers, compared to methylation insensitive primers, and showed a much higher conservation of wheat parental bands. Furthermore, the hexaploid wheat genomes were more conserved in triticales as compared to the tetraploid wheat genomes. However, more than 70 percent of the rye parental AFLP bands were absent in triticales and wheat/rye addition lines regardless of the materials and primers used. The RFLP analyses with 50 oat EST clones indicated that almost 100 percent of the EST bands that appeared in wheat also appeared in triticale, whereas only about 60 percent of the EST bands that appeared in rye were present in triticale. The overall results suggested that, when placed in wheat backgrounds, rye coding or non-coding sequences were involved in a very high level of variation regardless of whether it was induced by the addition of single rye chromosome or the entire rye genome.