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United States Department of Agriculture

Agricultural Research Service

Title: Possibilities for Calibration Transfer Across Diverse Instrumentation Platforms

Authors
item Barton Ii, Franklin
item Tseng, Ching-Hui - COGNIS CORP.
item Sohn, Mi Ryeong - VISITING SCIENTIST
item Himmelsbach, David
item Wang, Qian - BRUKER OPTICS

Submitted to: United States-Japan Cooperative Program in Natural Resources
Publication Type: Proceedings
Publication Acceptance Date: December 15, 2003
Publication Date: January 6, 2004
Citation: Barton II, F.E., Tseng, C., Sohn, M., Himmelsbach, D.S., Wang, Q. 2004. Possibilities for calibration transfer across diverse instrumentation platforms. United States-Japan Cooperative Program in Natural Resources, p.168-172.

Interpretive Summary: The most difficult task in utilizing spectroscopy and chemometrics for the determination of quality in agricultural products and foods is the transfer of calibrations from one instrument to another. This is difficult to maintain when all the instruments in a network are identical in make and model. When the platforms are different it is considerably more difficult. This study shows the extremes a calibration on a transmission 850-1050 nm dispersive instrument transferred to a full spectrum Ft-interferometer. The model can be transferred with a standard error of performance (SEP) of 0.195.

Technical Abstract: The current regulatory system for measuring protein content in wheat is based on calibrations of Foss/Tecator transmission near infrared instruments that scan the region from 850-1050 nm in 2 nm increments. This produces a 100 data point spectra, which is suitable for protein, moisture and oil calibrations. As this system of instruments becomes older, some are approaching 20 years of age; the need for more versatile instruments as well as replacements is becoming critical. The ideal solution would be to transfer calibrations to modern instruments with capabilities for determining the historically required analytical results and newer requirements for determining functional properties of agricultural commodities and foods. A study was conducted to test the efficacy of calibration transfer from the Tecator Infratec dispersive instrument to a modern FT-NIR from Bruker Optics. A file of spectra from 2203 wheat samples was transferred to a Bruker Matrix - F (Bruker Optics, Billerica MA 01821) and an RMSECV of 0.195% protein and an RMSEP of 0.22% protein obtained.

Last Modified: 12/18/2014
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