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United States Department of Agriculture

Agricultural Research Service

Title: Isolation and Characterization of Humic Substances from Soils Collected at Juniper Butte Range, Idaho

Authors
item Cary, T - USACRREL
item Palazzo, A - USACRREL
item Clapp, Charles
item Senesi, N - UNIV. OF BARI
item Clapp, J - UNIV. OF MN
item Tatinclaux, M - USACRREL

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2003
Publication Date: November 1, 2003
Citation: Cary, T.J., Palazzo, A.J., Clapp, C.E., Senesi, N., Clapp, J.J., Tatinclaux, M.L. 2003. Isolation and characterization of humic substances from soils collected at juniper butte range, idaho. Agronomy Abstracts. Paper No. A02-cary589283-Poster.

Technical Abstract: Naturally occurring humic substances in soils hold promise as a factor related to restoring native plants. We have characterized the humic acids (HAs) of the Juniper Butte Range (JBR) soils, in Idaho, and determined carbon compounds that may promote the establishment of slickspot peppergrass (Lepidium papilliferum) seedlings. Research included isolation and characterization of JBR soils in terms of HA content and relating this information to soil type and climate. Humic acids were extracted using a modified IHSS method, and isolated by membrane filtration, precipitated, dialyzed, and freeze-dried. Elemental analyses showed minor differences between HAs, with surface samples having higher C and N values. Differences were also noted between sample sites for carbohydrates, amino acids, and acidity components. FTIR, fluorescence, and CPMAS 13C-NMR spectra provided valuable characterization and compositional information.

Last Modified: 8/30/2014
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