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United States Department of Agriculture

Agricultural Research Service

Title: Lipid Composition of Karenia Brevis Blooms in the Gulf of Mexico

Authors
item Leblond, Jeffery - MID TENNESSEE ST UNIV, TN
item Evens, Terence
item Chapman, Peter - US EPA, FL

Submitted to: National Symposium on Harmful Marine Algae in the U.S.
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2003
Publication Date: December 9, 2003
Citation: Leblond,J.D.,Evens,T.J.,Chapman,P.J.2003.Lipid composition of karenia brevis blooms in the gulf of mexico. National Symposium on Harmful Marine Algae in the U.S.

Technical Abstract: In the Gulf of Mexico, recurring Karenia brevis blooms lead to significant health and economic impacts. K. brevis is one member of a small group of dinoflagellates, related morphologically and by DNA-based phylogenetic analysis, that synthesize the carotenoid, gyroxanthin diester, in place of the more widely distributed peridinin. While this novel photopigment has been proposed as a biomarker, especially for remote-sensing imaging technologies, to detect the emergence of K. brevis blooms, other chemicals such as sterols and triglycerides, respectively, with potential to report the distribution and physiological condition of K. brevis are required. Recent work from our laboratories characterizing the lipids of dinoflagellates has confirmed that K. brevis, together with those its close relatives, Karenia mikimotoi and Karlodinium micrum, lacking peridinin, possesses a relatively simple sterol profile comprised of two unusual primary 4-methyl sterols, (24S)-4a-methyl-5a-ergosta-8(14),22-dien-3b-ol (ED) and its 27-nor derivative (NED). An October 1999 K. brevis bloom in the waters of the northwest Gulf of Mexico provided an opportunity to examine the usefulness of these sterols and other lipids as indicators of K. brevis in phytoplankton communities. Lipid extracts of filtered bloom samples, fractionated to separate free and esterified sterols, were examined by GC/MS of trimethylsilyl ether derivatives. ED and NED were the major sterols found in all bloom samples. Fatty acids found in lipid fractions containing membrane phospholipids, chloroplast-associated glycolipids, and storage triglycerides, respectively, differed significantly. The glycolipid fraction was found to contain octadecapentaenoic acid [18:5(n-3)], a fatty acid commonly associated with dinoflagellates. The phospholipid fraction was found to contain small amounts of the recently described highly-unsaturated fatty acids, octacosaoctaenoic acid [28:8(n-3)] and octacosaheptaenoic acid [28:8(n-6)]. Fatty acids from the triglyceride fraction were more abundant than those associated with glycolipids and phospholipids. These results were found to closely resemble cultured K. brevis. They will be compared to a more recent Fall 2002 bloom.

Last Modified: 10/22/2014
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