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United States Department of Agriculture

Agricultural Research Service

Title: Identification and Cloning of the Gene Encoding Bmpc: An Outer Membrane Lipoprotein Associated with Brachyspira Pilosicoli Membrane Vesicles

item Alt, David
item Zuerner, Richard
item Bulach, D - MONASH UNIV, AS
item Wannemuehler, M - IOWA STATE UNIVERSITY
item Stasko, Judith
item Townsend, K - UNIV QUEENSLAND, AS
item Stanton, Thaddeus

Submitted to: Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 15, 2003
Publication Date: April 1, 2004
Citation: Trott, D.J., Alt, D.P., Zuerner, R.L., Bulach, D.M., Wannemuehler, M.J., Stasko, J.A., Townsend, K.M., Stanton, T.B. 2004. Identification and cloning of the gene encoding BmpC: an outer membrane lipoprotein associated with Brachyspira pilosicoli membrane vesicles. Microbiology. 150 (Pt 4):1041-1053.

Interpretive Summary: Intestinal bacteria known as spirochetes, in the genus Brachyspira, can cause serious diseases in livestock. Little is known about the bacterium B. pilosicoli that causes intestinal spirochetosis in swine and avian species. The goal of these experiments was to identify proteins located on the surface of this bacterium and that are useful targets for vaccine development and for developing diagnostic tests. A biochemical procedure was devised to isolate proteins from B. pilosicoli cell surfaces. Antibody reagents to detect specific proteins were prepared and shown to react with surface proteins. The gene for one abundant surface protein was cloned and analyzed. These experiments provide a foundation for identifying virulence associated surface proteins of this important pathogen and have generated immunological reagents that will allow us to characterize these bacteria during disease.

Technical Abstract:

Last Modified: 8/27/2014
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