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United States Department of Agriculture

Agricultural Research Service

Title: Engineering Synthetic Transcriptional Enhancer Cassettes

Authors
item Cazzonelli, Christopher
item Velten, Jeffrey

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Other
Publication Acceptance Date: July 25, 2003
Publication Date: July 25, 2003
Citation: Cazzonelli, C.I., Velten, J.P. 2003. Engineering synthetic transcriptional enhancer cassettes. American Society of Plant Biologists Annual Meeting.

Technical Abstract: Plant viruses have provided many of the strongest transcriptional promoters used to drive transgene expression in genetically modified plants. Based upon the observation that promoter control elements are often duplicated within highly active promoters, the intragenic regions from multiple, completely sequenced, plant viral genomes were scanned for repeated sequence motifs. The identified sequences were fused to a minimal CaMV35s promoter-luciferase reporter gene and screened for their ability to enhance luciferase activity using an Agrobacterium-based, in vivo, transient assay system optimised in tobacco leaves. Sequence elements that demonstrate enhancer activity were multimerised and/or combined to create novel transcriptional enhancer cassettes. Research currently focuses on determining the relative strength and expression characteristics of synthetic promoters containing the enchancer cassettes. Aside from providing new tools for the expression and control of transgene expression in plants, analysis of the identified sequence elements will provide additional insights into plant and viral transcriptional control mechanisms.

Last Modified: 10/21/2014
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