Technical Abstract: Garlic (Allium sativum L.) has been clonally propagated for hundreds of years because it does not produce seed under cultivated conditions. A single garlic accession frequently displays a high degree of phenotypic plasticity that is likely dependent upon soil type, moisture, and cultural practices. This diversity observed by growers has occasionally led to the renaming of varieties as they are exchanged among growers and gardeners. As a result, there are numerous garlic varieties available within the USDA National Plant Germplasm System and commercially that are very similar genetically, yet have unique varietal names. We have performed amplified fragment length polymorphism (AFLP) analysis on more than 200 accessions within the NPGS as well as many commercially available varieties to determine the extent of accession duplication among cultivated garlics. These data can be used to determine phylogenetic relationships among garlic varieties. Growers can also use these data to identify unique accessions within the NPGS that could be provided to the garlic growing community.