Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: August 15, 2003
Publication Date: November 1, 2003
Citation: Cotty, P.J., Taylor, D.R. 2003. Influence of complementation medium composition on vegetative compatibility analyses of Aspergillus flavus [abstract].Proceedings of the American Phytopathological Society Annual Meeting. S18. Technical Abstract: Reactions between complementary nit (nitrate non-utilizing) mutants of Aspergillus flavus were influenced by the composition of the medium on which vegetative compatibility analyses were performed. Vegetative compatibility groups were found to differ in their sensitivity to medium composition. Medium components that influenced complementation included simple sugars and complex polymers. Complementation was inhibited by reduced agar concentration (regardless of medium hardness), sucrose and fructose, and stimulated by starch and several other polymers. An improved medium for vegetative compatibility analysis of A. flavus was developed. The medium contained the following per liter: 3 g NaNO3, 1 g K2HPO, 0.5 g MgSO47H20, 0.5 g KCI, 36 g glucose, 20 g starch (Difco), 13 g agar (EM purified), micronutrients (optional). The medium was adjusted to pH 6.0 prior to autoclaving. In addition to support of faster analyses, the improved medium helped to resolve ambiguous reactions, simplify vegetative compatibility grouping, and expand the number of A. flavus VCGs that could be tracked with vegetative compatibility analyses.