Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 14, 2003
Publication Date: June 1, 2003
Citation: PALUMBO, J.D., KANEKO, A., GORSKI, L.A. DIFFERENTIAL DISPLAY OF LISTERIA MONOCYTOGENES TRANSCRIPTION DURING ATTACHMENT TO CABBAGE. AMERICAN PHYTOPATHOLOGICAL SOCIETY ANNUAL MEETING. 2003. v. 93 (6). (Suppl. 69). Technical Abstract: Listeria monocytogenes is a ubiquitous soil bacterium and an important foodborne human pathogen. Contamination of fresh produce may be an ecological transition between the soil environment and the human host; however, little is known regarding specific Listeria-plant interactions. To identify mechanisms involved in contamination of produce, we are investigating L. monocytogenes gene expression during attachment and growth on cabbage. We performed reverse transcription-PCR with 81 arbitrary primers and used differential display polyacrylamide gels to identify genes that show higher expression in L. monocytogenes cells recovered from cabbage surfaces relative to cells grown in defined medium. Several of these genes encode proteins with no known function. We are assessing the contribution of these proteins to plant colonization by measuring attachment of site-directed mutant L. monocytogenes strains relative to that of wild type L. monocytogenes.