ARS | Publication request: TOLL-LIKE RECEPTOR 2 AND ACUTE PHASE CYTOKINE RESPONSES BY GENETICALLY SELECTED CHICKENS FOLLOWING AN LPS CHALLENGE

Submitted to: Journal of Immunology
Publication Type: Abstract Only
Publication Acceptance Date: November 1, 2002
Publication Date: April 13, 2003
Citation: EICHER, S.D., CHENG, H. TOLL-LIKE RECEPTOR 2 AND ACUTE PHASE CYTOKINE RESPONSES BY GENETICALLY SELECTED CHICKENS FOLLOWING AN LPS CHALLENGE. JOURNAL OF IMMUNOLOGY. 2003. P. 28.

Technical Abstract: The objective of this study was to determine expression of TLR2 and two acute phase cytokines following an LPS challenge of two genetic lines of layers divergently selected for aggression (aggressive, MBB and passive, KGB)and one commercial genetic line (COMM). The 3 lines of layers were given LPS (5.0 mg/kg of body weight), then organs weighed and tissues collected at 0, 24, and 72 hours following a single LPS i.v. injection. RNA was extracted from the tissues and subjected to real-time RT-PCR. Heart and spleen weights had genetic line (P<0.01) and time effects (P<0.05 and 0.01, for heart and spleen respectively). Liver weights were different for genetic line (P<0.01) but not time. Liver IL-1 and TLR2 had time effects (P<0.01) but were not different among treatments. Liver TNF was not different over time or among treatments. Lung IL-1, TLR2, and TNF were different over time (P<0.01) but not among treatments. Spleen IL-1, TLR2, and TNF were different over time but not among treatments (P<0.05 for IL-1, P<0.01 for TLR2 and TNF). No treatment by times interactions were detected for any of these variables (P>0.10). The only effects of genetic line were liver and spleen weight. TLR2 and cytokine changes were not influenced by genetic lines, only by time. Therefore, this genetic selection has not altered RNA expression of these innate immune functions.