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United States Department of Agriculture

Agricultural Research Service

Title: Permanent Mapping Population of the Cotton Genome

Authors
item Yu, John
item Kohel, Russell

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: November 10, 2002
Publication Date: November 10, 2002
Citation: Yu, J., Kohel, R.J. 2002. Permanent mapping population of the cotton genome. American Society of Agronomy Meetings. 2002 CDROM.

Technical Abstract: Genetic mapping resource developed from an appropriate cross is the foundation for any genome mapping efforts. Lacking in Gossypium has been the availability of a recombinant inbred line (RIL) population that can be shared with the cotton research communtiy. We have developed 191 RILs from the cross of TM-1 x3-79, the genetic standards for G. hirsutum and G. barbadense, respectively. Extensive genetic and cytogenetic stocks were derived from the two cottons, including a set of aneuploid stocks for rapid localization of genetic markers to cotton chromosomes. The RILs (F7) of the above population were developed via single seed decent (SSD) from the original F2 individuals from which a genetic map was constructed, consisting of approximately 1,000 DNA markers (RFLPs, RAPDs, AFLPs, and SSRs). Characterization of the RILs with a set of the above markers indicates that 95% of genetic loci are fixed in the population and they behave normally in the cotton genomel. The RIL population retains the phenotypic variability of the original F2. We are now increasing this permanent mapping population for distubution to the cotton research community for integrated genome mapping.

Last Modified: 7/25/2014
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