Technical Abstract: Sugar beet is an important agronomic crop that exhibits considerable genetic variation for morphological and physiological traits. Currently there is only a limited set of publicly available microsatellite markers for use in beet. The objectives of this work were to identify additional (simple sequence repeat) SSR markers from genomic libraries of sugar beet and test their efficacy in differentiating several beet accessions. A set of 8 microsatellite loci is presented. Sequences for representative alleles show several different repeat motifs ranging from simple di-nucleotide repeats to more complex compound repeats with both tetra and tri-nucleotide repeats. These 8 loci show very high heterozygosity levels (38 alleles scored) and differentiate a cultivated sugar beet (FC712), wild sea beet (Beta vulgaris var. maritima) and an accession of garden beet (Fst between accession =0.452).