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United States Department of Agriculture

Agricultural Research Service

Title: Total Fat Analysis in Milk-Based and Soy-Based Infant Formula Powder by Supercritical Fluid Extraction (Sfe)

Authors
item Wolf, Wayne
item Lacroix, Denis
item Goel, Ritu

Submitted to: Journal of the American Oil Chemists' Society
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 31, 2003
Publication Date: February 28, 2003
Citation: Wolf, W.R., Lacroix, D.E., Goel, R. 2003. Total fat analysis in milk-based and soy-based infant formula powder by supercritical fluid extraction (sfe). Journal of the American Oil Chemists' Society. 80:(9) 853-857.

Interpretive Summary: A rapid automated method for the determination of total fat in infant formula powders using a commercially available Supercritical Fluid Extraction (SFE) instrument was developed and evaluated. The matrices examined were Standard Reference Material SRM 1846 Infant Formula (NIST) and commercial milk-based and soy-based infant formula powders. Method verification and validation was done by linear regression analysis using the Method of Standard Additions (MOSA). The Data Quality Objectives (DQO) format was used to define and evaluate the characteristic parameters of instrumental performance. A peer validation study between three participating laboratories showed excellent agreement with the label declared percent fat values and excellent reproducibility. Linear regression analysis of all infant formula matrices spiked with added fat shows no observed interferences in the SFE instrument response. Integrating the DQO process with a readily available certified reference material, and demonstrating reproducibility by two outside collaborating laboratories, validates the accuracy of the data obtained by this method. Food testing and analysis laboratories in the public and private sector will use this method to replace present methods that use hazardous solvents.

Technical Abstract: A rapid automated method for the determination of total fat in infant formula powders using a commercially available Supercritical Fluid Extraction (SFE) instrument was developed and evaluated. The matrices examined were Standard Reference Material SRM 1846 Infant Formula (NIST) and commercial milk-based and soy-based infant formula powders. Method verification and validation was done by linear regression analysis using the Method of Standard Additions (MOSA). The Data Quality Objectives (DQO) format was used to define and evaluate the performance characteristic parameters of instrumental total fat analysis by SFE. A peer validation study showed excellent agreement with the label declared percent fat values, and excellent reproducibility among three laboratories. Linear regression analysis of all infant formula matrices spiked with added fat shows that the SFE instrument response is due to the added analyte only with no observed interferences. Integrating the DQO process with a readily available certified reference material, and showing reproducibility by two outside collaborating laboratories, establishes validation of the accuracy of the data obtained by this method.

Last Modified: 8/29/2014
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