Submitted to: International Association for Plant Tissue Culture & Biotechnology Congress
Publication Type: Abstract Only
Publication Acceptance Date: June 23, 2002
Publication Date: June 23, 2002
Citation: OW, D.W. RECOMBINASE-BASED METHODS TO INTEGRATE, STACK AND TRANSLOCATE DNA IN PLANTS. INTERNATIONAL ASSOCIATION FOR PLANT TISSUE CULTURE & BIOTECHNOLOGY CONGRESS. June 28-28 2002. Technical Abstract: The exact placement of foreign DNA into the plant genome produces transgenes with greater structural fidelity and faithful expression. Recombinase-mediated site-specific integration has been reported for tobacco, Arabidopsis, maize and rice. The next challenge will be to develop practical strategies to stack and translocate DNA. Being able to append new DNA sequentially to a target site permits the continual use of a previously characterized chromosome location, which justifies the initial investment costs in identifying favorable chromosome targets. Stacking transgenic traits at a limited number of target sites is also preferable to scattering transgenes all over the genome, as the clustering of transgenes expedites the introgression of bundled traits to field cultivars. Strategies can also be developed to translocate DNA between chromosomes to reduce the linkage drag in the introgression of transgenes into elite lines. This presentation will discuss methods for inserting, stacking and translocating DNA into a chromosome target.