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United States Department of Agriculture

Agricultural Research Service

Title: Effect of Selenium-Enriched Broccoli Diet on Differential Gene Expression in Min Mice Liver

Authors
item Zeng, Huawei
item Davis, Cindy
item Finley, John

Submitted to: Journal of Nutritional Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 20, 2002
Publication Date: April 1, 2003
Repository URL: http://handle.nal.usda.gov/10113/2482
Citation: Zeng, H., Davis, C.D., Finley, J.W. 2003. Effect of selenium-enriched broccoli diet on differential gene expression in min mouse liver. Journal of Nutritional Biochemistry 14:227-231.

Interpretive Summary: The essential nutritional role of selenium (Se) in animals and humans is well established. Previous studies have shown that selenium-enriched broccoli reduces colon cancer susceptibility effectively. Because colon cancer cells mainly metastasize to the liver, the study of effects of selenium-enriched broccoli on cancer related gene expression in the liver was undertaken to further our understanding of the protective role for selenium-enriched broccoli in tumorigenesis. This study investigated the effects of selenium-enriched broccoli on the binding of important liver transcription factors to their cis-acting elements, which is the essential step in the control of gene expression. Our results suggest that selenium-enriched broccoli enhances the binding of numerous transcription factors particularly p53 (a tumor suppressor gene product) to cis-acting elements. The data may extend the understanding of the anti-cancer effect of selenium-enriched broccoli. These findings will be useful for scientists and health-care people who study food and cancer prevention.

Technical Abstract: Previous studies have shown that selenium-enriched broccoli effectively reduces colon cancer susceptibility. Because colon cancer cells mainly metastasize to the liver, studying the effects of selenium-enriched broccoli on cancer-related gene expression in the liver might increase our understanding of the protective role for selenium-enriched broccoli in tumorigenesis. This study determined the effects of selenium-enriched broccoli on the binding of important liver transcription factors to their cis-acting elements, which is essential in the control of gene expression. Our results suggest that selenium-enriched broccoli enhances the binding of numerous transcription factors particularly p53 protein (a tumor-suppressive transcription factor) to cis-acting elements. These observations may have additional implication for understanding of an anti-cancer effect of selenium-enriched broccoli.

Last Modified: 4/16/2014
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