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United States Department of Agriculture

Agricultural Research Service

Title: Effect of Feeding the Ionophores Monensin and Laidlomycin Propionate and the Antimicrobial Bambermycin to Sheep Experimentally Infected with E. Coli 0157:h7 and Salmonella Typhimurium

Authors
item Edrington, Thomas
item Callaway, Todd
item Bischoff, Kenneth
item Genovese, Kenneth
item Elder, Robert
item Anderson, Robin
item Nisbet, David

Submitted to: Journal of Animal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 14, 2002
Publication Date: February 1, 2003
Citation: Edrington, T.S., Callaway, T.R., Bischoff, K.M., Genovese, K.J., Elder, R.O., Anderson, R.C., Nisbet, D.J. Effect of feeding the ionophores monensin and laidlomycin propionate and the antimicrobial bambermycin to sheep experimentally infected with E. coli 0157:H7 and salmonella typhimurium. Journal of Animal Science. 2003. v. 81. p. 553-560.

Interpretive Summary: Ionophores are antibiotics that improve the growth and performance of young cattle and sheep. The use of ionophores in cattle production is very common. The similar time frame in which ionophore use began and food sickness caused by E. coli O157:H7 has raised concerns. The purpose of the present study was to determine if feeding ionophores has any impact on E. coli and Salmonella in sheep. Results showed no effect of short- term ionophore feeding on fecal shedding or of gut concentrations of E. coli or Salmonella. Likewise, no effects were seen on the development of antimicrobial resistance in these bacteria.

Technical Abstract: Escherichia coli O157:H7 and Salmonella are widely recognized as important agents of food-borne disease with worldwide distribution. The use of ionophores in feeding growing ruminants is widespread in the United States and has attracted recent interest due to the apparent temporal relationship between initial ionophore use and the increase in human E. coli O157:H7 cases. Two experiments were conducted to evaluate the effects of short-term feeding of ionophores on fecal shedding, intestinal concentrations, and antimicrobial susceptibility of E. coli O157:H7 and S. Typhimurium in growing lambs. Sixteen lambs were used in each experiment, four lambs per treatment group: monensin (MON), laidlomycin propionate (LP), bambermycin (BBM) and a control (CON) treatment. Lambs were fed a grain and hay diet with their respective ionophore for 12 days prior to experimental inoculation with a non-toxigenic strain of E. coli O157:H7 or S. Typhimurium. Animals continued on their respective diets an additional 12 days and fecal shedding was monitored daily. Lambs were euthanized and tissues and contents sampled from the rumen, cecum, and rectum. No differences (P > 0.05) in fecal shedding of Salmonella or E. coli were found, although E. coli concentration appeared to decrease more quickly over time in control animals compared with ionophore treated animals. Lumen contents and tissue samples from the rumen, cecum and rectum for Salmonella or E. coli were not different (P > 0.05) among treatments. Feeding MON did decrease (P < .05) the incidence of scours in sheep infected with Salmonella when compared to CON, BBM, and LP treatments. No differences in anti-microbial susceptibility were found in any of the E. coli or Salmonella isolates. These studies found no significant effects of short-term ionophore feeding on E. coli and Salmonella shedding or on anti-microbial susceptibility in experimentally infected lambs.

Last Modified: 4/18/2014
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