Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 26, 2002
Publication Date: August 26, 2002
Technical Abstract: BVDV2 strains can cause disease ranging from clinically inapparent to severe acute hemorrhagic syndrome. Severe acute (SA) BVD is caused by the virulent members of the BVDV2 genotype. SA BVD is characterized by fever (>/= 106 C), thromobocytopenia (>/= 60% decrease in circulating platelets), lymphopenia (>/= 60% decrease in circulating lymphocytes), neutropenia and often death. Noncytopathic strains of BVDV2, including those that cause SA BVD, have the ability to establish persistent infections in calves infected in the first 150 days of pregnancy. The mechanisms of virulence and development of persistence are unknown. To better understand the interactions of the host cell and the virus in both acute disease and persistence, serial analysis of gene expression (SAGE) was used to develop a global picture of transcriptional changes that take place in Madin Darby bovine kidney (MDBK) cells following infection with the virulent BVDV2 strain 1373. SAGE, a sequence based, global functional genomics technology, allows quantitation of virtually every transcript in a cell type without prior sequence information. Transcript expression levels and identities are determined by DNA sequencing of libraries composed of 14 base DNA fragments (tags) derived from the 3' end of each cellular mRNA transcript. A total of 26,611 tags from the noninfected control MDBK library and 26,457 tags from the BVDV2 1373-infected MDBK library were sequenced. Comparison of the SAGE data obtained from noninfected and BVDV2-infected MDBK cells revealed that 72 genes were downregulated and 79 genes were upregulated (>/= 5-fold) in BVDV2-infected cells where there were >/= 8 total tags representing a transcript. Many, but not all of the changes in gene expression that were observed were beneficial to the virus while placing the cell at a metabolic disadvantage.