Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 13, 2002
Publication Date: December 31, 2002
Citation: Lee, L.F. 2002. Influence of b-haplotype on the efficacy of recombinant fowlpox vaccine protection against marek's disease in chickens [abstract]. American Veterinary Medical Association. p. 19. Technical Abstract: Recombinant fowlpox virus (rFPV) containing glycoprotein gB genes from three serotypes of Marek's disease virus (MDV) was used to study the influence of B-haplotype on vaccine responses in chickens. Sequence analysis of the gB gene from three serotypes showed 80% homology. Chickens were vaccinated intraperitoneally at hatch. At 6 days of age, chickens were challenged with 500 pfu of strain Md5 of MDV. The level of protection by gB of three serotypes was significantly different in B congenic lines of chickens. The level of protection in chickens was highest for B*21/*21, intermediate for B*13/*13 and lowest for B*5/*5. In two additional trials, a rFPV containing four MDV genes was used to study the efficacy of protection in chickens of different B haplotypes. The result confirms that B*21/*21 chickens provide the best protection against challenge with several MDV viruses. These results taken together indicate a significant influence of B haplotypes on vaccine responses resulting from rFPV.