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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #133445
Title: CHARACTERIZATION OF THIRTY EIGHT POLYMORPHIC MICROSATELLITE MARKERS FOR RAINBOW TROUT (ONCORHYNCHUS MYKISS)

Author
item Palti, Yniv
item Fincham, Michele
item Rexroad, Caird

Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2002
Publication Date: 12/1/2002
Citation: Palti, Y., Fincham, M.R., Rexroad Iii, C.E. 2002. Characterization of thirty eight polymorphic microsatellite markers for rainbow trout (oncorhynchus mykiss). Molecular Ecology Notes. 2(4):449-452.

Interpretive Summary: Efforts to use molecular technologies in the genetic improvement of agricultural species have recently included the genetic mapping of rainbow trout. This report gives details on 38 new genetic markers developed for mapping in rainbow trout. This is the sixth set of markers from the marker development phase of our mapping project.

Technical Abstract: Thirty-eight new microsatellite markers have been developed for genome mapping and population genetics studies in rainbow trout (Oncorhynchus mykiss). The amount of polymorphism, percent heterozygosity, and ability of each marker to amplify genomic DNA from other salmonids was also observed. Some of the markers were observed to be duplicated in the rainbow trout genome by containing more than one allele in homozygous (clonal) fish.