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United States Department of Agriculture

Agricultural Research Service

Title: Phylogenetic Analyses of Magnaporthe Grisea Based on Internal Transcribed Spacer (Its) and Translation Elongation Factor (Tef) Sequences

item Goodwin, Stephen
item Levy, Morris - PURDUE UNIVERSITY

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: July 29, 2002
Publication Date: N/A

Technical Abstract: Rice blast is caused by Magnaporthe grisea and it is the most important disease of rice in the world. Due to its economic significance, M. grisea has become the model pathogen for molecular plant pathology research. Its pathogenicity has been studied extensively. However, the phylogenetic relationships of M. grisea are less studied. Previous studies on M. grisea populations have focused on its host diversity, DNA fingerprint and RFLP variation. We performed phylogenetic analyses based on sequences of its ribosomal DNA region (ITS1, ITS2 and 5.8S gene) and intron sequences of TEF alpha. Twenty-four isolates of M. grisea from different geographical places and hosts were analyzed for ITS and TEF sequence. Similar sequences were downloaded from GenBank and incorporated into the phylogenetic tree. We have found several distinct subgroups among these 24 isolates. We also suggest that Magnaporthe poae may be more related to Gaeumannomyces than to Magnaporthe based on its ITS sequence downloaded from GenBank.

Last Modified: 4/19/2015
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