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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Insect Genetics and Biochemistry Research » Research » Publications at this Location » Publication #132613
Title: CLONING OF THREE DESICCATION REGULATED TRANSCRIPTS FROM DIAPAUSING COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA

Author
item Yocum, George

Submitted to: Molecular Insect Science International Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/27/2002
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Clones of three desiccation up-regulated transcripts (LdDes-1, -2 & -3) were isolated from diapausing Colorado potato beetle using suppression subtractive hybridization. Northern blot analysis demonstrated that LdDes-1, -2 & -3 are highly up-regulated in diapausing beetles and in desiccated nondiapausing adults. Transcript sizes are 1.35 Kb (LdDes-1), 0.8 Kb (LdDes-2) and 0.54 Kb (LdDes-3). A BlastX search revealed that LdDes-1 & -3 have identity to no known genes. The highest score for LdDes-2 was to the 28 kDa desiccation protein of Tenebrio molitor at 31% identity and 48% positives (identity + chemically equivalent). LdDes-1 encodes for a deduced protein of 34 kDa in size and is enriched (>9% each) for the amino acids glutamic acid, leucine, methionine, arginine and asparagine. The complete open reading frame for LdDes-3 has yet to be determined.