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United States Department of Agriculture

Agricultural Research Service

Title: Ability of An Elisa-Based Seed Health Test to Detect Erwinia Stewartii in Maize Seed Treated with Fungicides and Insecticides

Authors
item Michener, P - UNIV. OF ILLINOIS
item Pataky, J - UNIV. OF ILLINOIS
item Block, Charles
item Shepherd, L - IOWA STATE UNIV.
item Mcgee, D - IOWA STATE UNIV.

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: March 19, 2002
Publication Date: July 31, 2002
Citation: Michener, P.M., Pataky, J.K., Block, C.C., Shepherd, L.M., Mcgee, D.C. 2002. Ability of an ELISA-based seed health test to detect Erwinia stewartii in maize seed treated with fungicides and insecticides [abstract]. Phytopathology. 92:S56

Technical Abstract: An ELISA-based seed health test detected E. stewartii in infected maize seed treated with 11 combinations of captan, carboxin, clothianidin, imidacloprid, thiamethoxam, fludioxonil, mefenoxam, or thiram. Treated healthy seeds were blended with treated E. stewartii-infected seed of Jubilee or A632 to produce 100-kernel samples with a 96% and 88% chance, respectively, that a randomly-selected sample would contain infected kernels. Forty-six samples were tested per seed treatment, plus a non-treated control. Based on 5% Type I errors, >20 of 23 positive samples were expected per treatment in samples blended with Jubilee, and >17 of 23 positive samples were expected per treatment in samples blended with A632. For Jubilee, all treatments had >20 positive samples. For A632, all treatments had >17 positive samples except for seed treated with clothianidin or captan/carboxin which had 17 and 15 positive samples, respectively. Mean absorbance values of positive samples did not differ among seed treatments.

Last Modified: 7/25/2014
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