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United States Department of Agriculture

Agricultural Research Service

Title: The Study of Wheat Starch Size Distribution Using Image Analysis and Laser Diffraction Technology

item Bechtel, Donald

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 28, 2002
Publication Date: October 13, 2002
Citation: Wilson, J.D., Bechtel, D.B. 2002. The study of wheat starch size distribution using image analysis and laser diffraction technology. Abstract No. 213 in: 2002 AACC Annual Meeting Program Book. p.115. Meeting Abstract.

Interpretive Summary: Abstract for AACC Annual Meeting to be held October 13-17, 2002 in Montreal, Canada.

Technical Abstract: Starch is the most abundant storage reserve in the wheat caryopsis yet little is known about its influence on end-use properties. Starch was isolated from wheats of four different classes and analyzed using digital image analysis (IA) coupled to a light microscope and several laser diffraction sizing instruments (LDS). The IA data was converted into volume data in order to compare to LDS data. LDS analysis tended to underestimate both A and B starch granule populations when compared to IA. Linear correlations comparing IA to LDS instruments ranged from r = 0.17599 (p>0.1) to r = 0.73956 (p<0.001) depending on the LDS instrument used. A correction factor was developed to convert LDS starch size distribution data to that obtained using IA. The corrections were validated to four classes of wheat; Spelt, Hard Red Winter (HRW), Hard Red Spring (HRS), and Durum. R2 values of the corrected starch size distribution general linear regression model were as follows: Spelt- 0.86, HRW- 0.77, HRS- 0.79, and Durum- 0.89. The corrections will be used to develop a standard method of analysis for measuring wheat starch size distributions quickly and accurately.

Last Modified: 4/22/2015
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