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United States Department of Agriculture

Agricultural Research Service

Title: Isolation of An Effective Promoter from Citrus Yellow Mosaic Virus for Expression of Disease Resistant Genes in Ornamental Crops

Authors
item Huang, Qi
item Hartung, John

Submitted to: BARC Poster Day
Publication Type: Other
Publication Acceptance Date: April 15, 2002
Publication Date: N/A

Technical Abstract: Bacterial diseases caused by Agrobacterium, Erwinia, Pseudomonas, Xanthomonas and Ralstonia spp. often result in significant loses in the production and quality of ornamental crops, and are very difficult to control. The use of genetically engineered crops offers a novel, cost effective and environmentally sound strategy for control of bacterial diseases. Commercialization of such crops, however, is limited by the use of promoters such as the widely studied 35S promoter from Cauliflower mosaic virus (CaMV) that have licensing restrictions. To obviate licensing problems, we isolated a promoter fragment from a cloned isolate of Citrus yellow mosaic virus (CYMV), a double-stranded DNA plant virus like CaMV. This was done by fusing different regions fo the CYMV genome to the coding region of the B-glucronidase (GUS) gene and a CYMV region with strong promoter activity was identified by analyzing the amount of transient GUS gene expression in tobacco leaves transformed by particle bombardment. We are in the process of comparing the strength of the CYMV promoter with that of the CaMV 35S promoter and a duplicated CaMV 35S promoter. The utilization of the CYMV promoter for gene expression in ornamental crops will be discussed. These studies are important steps toward the development and successful commercialization of transgenic ornamental crops for bacterial resistance.

Last Modified: 11/27/2014
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