ARS | Publication request: CLONING AND SEQUENCING OF A FULL-LENGTH CDNA ENCODING FOR PHOSPHOLIPASE D IN PEANUT.

Submitted to: Aflatoxin Elimination Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: December 31, 2001
Publication Date: March 1, 2002
Citation: Guo, B., Xu, G., Holbrook, Jr., C.C., Lynch, R.E. 2002. Cloning and sequencing of a full-length CDNA encoding for phospholipase D in peanut[abstract]. Mycopathologia. 155:91.

Technical Abstract: Preharvest aflatoxin contamination has been identified by the peanut industry as the most serious challenge facing the industry worldwide. Drought stress is the most important environmental factor exacerbating Aspergillus infection and aflatoxin contamination in peanut. Development of resistant peanut cultivars would represent a major advance for the U.S. peanut industry. In this study, we identify a novel PLD gene, encoding a putative phospholipase D, a main enzyme responsible for the drought-induced degradation of membrane phospholipids in plants. The completed cDNA sequence was achieved by using the consensus- degenerate hybrid oligonucleotide primer strategy. We have used the sequence information encoded by the cloned fragments to amplify both the 5' and 3' ends of this gene to obtain a full length clone. The deduced amino acid sequence shows high identity with known PLD genes, having similar conserved features, such as two HXKXXXXD motifs. Further study is needed to genetically and physiologically characterize the PLD in peanut and to gain a better understanding of its function and relationship with drought-tolerance.