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United States Department of Agriculture

Agricultural Research Service

Title: Rflp Assessment on Heterogeneous Nature of Maize Population Gt-Mas:gk and Field Evaluation of Resistance to Aflatoxin Production by Aspergillus Flavus.

item Guo, Baozhu
item Widstrom, Neil
item Lynch, Robert

Submitted to: Aflatoxin Elimination Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: June 1, 2001
Publication Date: January 1, 2002
Citation: Butron, A., Li, H., Guo, B., Widstrom, N.W., Lynch, R.E. 2002. RFLP assessment on heterogeneous nature of maize population GT-MAS:GK and field evaluation of resistance to aflatoxin production by aspergillus flavus [abstract]. Mycopathologia. 155:88.

Technical Abstract: The challenge to breeders/plant pathologists is to identify lines that have resistance to aflatoxin production. Maize population GT-MAS:gk has been identified and released as a germplasm with resistance to aflatoxin contamination. In the present study, we assessed genetic divergence in the GT-MAS:gk population using RFLP DNA markers to survey 11 selfed inbred lines and conducted field evaluations for the dissimilarities in aflatoxin production among these inbred lines in comparison with a sister population, GT-MAS:pw,nf. The 11 selfed inbred lines were assayed for DNA polymorphism using 113 restriction fragment length polymorphism (RFLP) markers in 10 linkage groups covering 1518.2 cM. Considerable variation among the inbreds was detected with RFLP markers of which 42 probe-enzyme combinations gave 102 polymorphic bands. Cluster analysis based on genetic similarities revealed associations and variations among the tested lines. Three polymorphic groups were distinguished by cluster analysis. A two-year field evaluation data showed that aflatoxin concentration among the lines were significantly different in both years (P< 0.001). The maturity data were also different. Thus, this study demonstrated that the maize population GT-MAS:gk is heterogeneous and individuals may be different in resistance to A. flavus infection and aflatoxin production. Therefore, the most resistant lines should be inbred to increase homogeneity and resistance should be confirmed through progeny testing.

Last Modified: 4/17/2015
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