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Title: GLYCOPROTEINS E AND I OF MAREK'S DISEASE VIRUS SEROTYPE (MDV-1) ARE ESSENTIAL FOR VIRUS GROWTH IN CULTURED CELLS

Author
item SCHUMACHER, DANIEL - FRIEDRICH-LOEFFLER INSTIT
item TISCHER, B. - FRIEDRICH-LOEFFLER INSTIT
item Reddy, Sanjay
item OSTERRIEDER, NIKOLAUS - FRIEDRICH-LOEFFLER-INSTIT

Submitted to: Journal of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/30/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary: Marek's disease (MD), a virus induced cancer-like disease of chickens, is considered as a major disease problem in commercial poultry. Vaccination has dramatically reduced the incidence of the disease, but very little is known about the basic mechanisms involved in the induction of disease. The objective of this research was to characterize two proteins that aid in the spread of this important poultry pathogen. The information shows how these two viral proteins aid in the spread of the virus from one living cell to another and will undoubtedly help scientists in academia and industry understand the spread of this virus and eventually lead to better control of the disease.

Technical Abstract: The role of glycoprotein E (gE) and gI of Marek's disease virus serotype 1 (MDV-1) for growth in cultured cells was investigated. MDV-1 mutants lacking either gE (20DeltagE), gI (20DeltagI), or both gE and gI (20DeltagEI) were constructed by recE/T-mediated mutagenesis of a recently established infectious bacterial artificial chromosome (BAC) clone of MDV-1 (D. Schumacher, B. K. Tischer, W. Fuchs, and N. Osterrieder, J. Virol. 74:11088-11098, 2000). Deletion of either gE or gI, which form a complex in MDV- 1-infected cells, resulted in the production of virus progeny that were unable to spread from cell to cell in either chicken embryo fibroblasts or quail muscle cells. This was reflected by the absence of virus plaques and the detection of only single infected cells after transfection, even after coseeding of transfected cells with uninfected cells. In contrast, growth of rescuant viruses, in which the deleted glycoprotein genes were reinserted by homologous recombination, was indistinguishable from that of parental BAC20 virus. In addition, the 20DeltagE mutant virus was able to spread from cell to cell when cotransfected into chicken embryo fibroblasts with an expression plasmid encoding MDV-1 gE, and the 20DeltagI mutant virus exhibited cell-to-cell spread capability after cotransfection with a gI expression plasmid. The 20DeltagEI mutant virus, however, was not able to spread in the presence of either a gE or gI expression plasmid, and only single infected cells were detected by indirect immunofluorescence.