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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #128709
Title: GENOMES, MULTIPLE ORIGINS, AND LINEAGE RECOMBINATION IN THE GLYCINE TOMENTELLA (LEGUMINOSAE) POLYPLOID COMPLEX: HISTONE H3-D GENE SEQUENCES

Author
item DOYLE, JEFF - CORNELL UNIVERSITY
item DOYLE, JANE - CORNELL UNIVERSITY
item BROWN, A H D - CSIRO, AUSTRALIA
item Palmer, Reid

Submitted to: Evolution
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/13/2002
Publication Date: 10/2/2002
Citation: DOYLE, J.J., DOYLE, J.L., BROWN, A., PALMER, R.G. GENOMES, MULTIPLE ORIGINS, AND LINEAGE RECOMBINATION IN THE GLYCINE TOMENTELLA (LEGUMINOSAE) POLYPLOID COMPLEX: HISTONE H3-D GENE SEQUENCES. EVOLUTION. 2002. V. 56. P. 1388-1402.

Interpretive Summary: Many plants have different "amounts" of genetic material within the same species. One example is a two-fold or more of the genetic material. Sometimes the genetic material is doubled (from 2n to 4n) or alternatively, two different species (or different forms of the same species) could cross-pollinate (2n x 2n to give 4n). In the wild perennial soybean species, Glycine tomentella, molecular studies of a protein (histone H3-D) of 4n plants was used to tentatively determine the 2n parents. Interestingly, sometimes the same 2n parents were identified in different 4n populations and could either be the male or the female parent. Also, 2n G. tomentellas may have been parents with different 2n Glycine species to produce 4n G. tomentellas. The various origins of 4n G. tomentella populations offer insight into Glycine evolution and speciation. Such information may be helpful to scientists to explain the origin of other species within the genus Glycine, e.g., the cultivated soybean G. max.

Technical Abstract: Several different taxa comprise Glycine tomentella, and relationships among polyploids and diploids have been hypothesized from crossing studies, isozyme data, and repeat length variation for the 5S nuclear ribosomal gene loci. However, several key questions have persisted, and detailed phylogenetic evidence from homoeologous genes has been lacking. The histone H3-D locus is single copy in diploid Glycine species, and has been used to elucidate relationships among diploid races of G. tomentella, providing a framework for testing genome origins in the polyploid complex. For all six G. tomentella polyploid races (T1-T6), alleles at two homoeologous histone H3-D loci were identified and their phylogenetic placement allowed all genomes to be categorized unambiguously and referred to alleles from diploid Glycine species. Allele phylogenies constructed for each allele group indicated multiple origins of several tetraploid races. Joint assessment of both homoeologous loci suggested lineage recombination following polyploid formation in some races. Most alleles in polyploids are identical or closely related to alleles in diploids, suggesting recency of polyploid origins and spread beyond Australia. These features parallel the other element of the Glycine subgenus Glycine polyploid complex, G. tabacina, one of whose races share a diploid genome with a G. tomentella race.