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United States Department of Agriculture

Agricultural Research Service

Title: Arsenic Deprivation in Rats Is Affected by Vitamin B12 Status

Author
item Uthus, Eric

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2001
Publication Date: March 20, 2002
Citation: Uthus, E.O. 2002. Arsenic deprivation in rats is affected by vitamin B12 status [abstract]. Federation of American Societies for Experimental Biology. 16:A270.

Technical Abstract: Arsenic (As) may have a role in methionine (MET) metabolism. To determine the role of As in MET metabolism, 2 experiments, using As-deprived (As-, <10 ng As/g) or supplemented (As+, 0.5 ug As as NaAsO2/g) rats, were carried out. MET metabolism was stressed by nitrous oxide (N2O) exposure (90 min/d for the last 15-18 d). N2O inactivates cobalamin and hence MET synthase (MS), a vitamin B12-dependent enzyme. In experiment 1 there was n vitamin B12 added to the basal diet; experiment 2, which used the same basal diet, contained B12 (25 ug/kg). After 60 d 2 of 8 As- rats died in experiment 1, 2 others were cold and lethargic; this was not seen in As+ rats or in As- or As+ rats from experiment 2. Significantly increased plasma BUN and GOT suggest that there was kidney or liver damage in the As- rats from experiment 1. No vitamin B12 was detected in the plasma of rats from experiment 1. As did not affect the activity of MS in either experiment; however, the activity was found to be considerably lower in experiment 1 compared to experiment 2. Thus, it appears that if vitamin B12 status is very low, As deprivation has severe consequences. This finding suggests an interaction between As and vitamin B12.

Last Modified: 7/31/2014
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