ARS | Publication request: ORAL ASCORBIC ACID AND BETA-GLUCAN AFFECT CYTOKINE EXPRESSION DIFFRERENTLY AFTER AN LPS CHALLENGE

Submitted to: Society for Leukocyte Biology Meetings Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 8, 2001
Publication Date: November 8, 2001
Citation: EICHER, S.D., MCKEE, C.A., CARROLL, J.A., JOHNSON, T.R. ORAL ASCORBIC ACID AND BETA-GLUCAN AFFECT CYTOKINE EXPRESSION DIFFRERENTLY AFTER AN LPS CHALLENGE. SOCIETY FOR LEUKOCYTE BIOLOGY MEETINGS PROCEEDINGS. 2001. V. 1. ABSTRACT P. 85.

Technical Abstract: Beta-glucan (Bg) from yeast and ascorbic acid (AA)can modulate innate immunity. Our objective was to investigate acute cytokine responses after a lipopolysaccharide (LPS) challenge in swine given an oral Bg product and an AA product. Pigs (n=8/trt) received carrier only (Control), a Bg product (2.5% of the diet), AA (75 ppm), or both (Both) from birth for 28 days, then LPS was delivered i.v. (150 ug/kg). Three hours after the LPS challenge, pigs were euthanized, a lung lavage used to obtain pulmonary alveolar macrophages (PAMs), and lung, spleen, and liver tissues collected and total RNA extracted for semi-quantitative RT-PCR. Interleukin-1 (IL-1) expression in PAMs was greater for pigs receiving BG than for the Both and Control pigs (P<0.05) and tended (P=0.06) to be greater than that of AA supplemented pigs. AA pigs had the least IL-1 expression in spleens (P<0.05) compared to BG and Both treatments and tended to be less (P=0.06) than Il-1 expression in the spleens of Control pigs. BG also increased IL-1 expression in the liver compared to the AA supplemented pigs (P< 0.05). IL-1 receptor antagonist (Ra) expression tended to increase with BG compared to Controls in PAMs and liver expression of IL-1Ra of pigs receiving Both tended to be greater than AA pigs (P< 0.10). TNF-alpha expression in lung tissue tended (P< 0.10) to be greatest for BG and Both pigs compared to AA and Control pigs. TNF- alpha expression was not detectable in PAMs, spleen cells, or liver cells. Bg and VC delivered together improved growth rates, but did not enhance or suppress acute cytokines 9which are necessary, but can be detrimental). This combination may be an enhancer of piglet well-being if antibiotics can not be given prophylactically.