|Rosewich, Liane - USDA CEREAL DISEASE LAB|
|Hernick, Charles - UNIVERSITY OF MINNESOTA|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: September 1, 2001
Publication Date: September 1, 2001
Citation: ROSEWICH, L.G., HERNICK, C.A., TAKAMURA, K., KISTLER, H.C. POPULATION GENETIC ANALYSIS OF FUSARIUM GRAMINEARUM LINEAGES 6 AND 7. INOCULUM 2001. Abstract v. 52 p. 56. and PHYTOPATHOLOGY. 2001. Abstract. v. 91. p. S122. Technical Abstract: While several studies have examined population diversity within Fusarium graminearum (teleomorph: Gibberella zeae), a causal agent of head blight of wheat and other cereals, so far, none have used a co-dominant marker system. We have identified nuclear RFLPs by screening a genomic library of single-insert clones. Screening of the initial 83 clones resulted in 12 probes, which are polymorphic within lineage 7 (U.S. isolates) or lineage (isolates from China). Interestingly, no repetitive probes have been encountered so far, suggesting that transposable elements or other middle or highly repetitive sequences are relatively rare in this species. The 12 polymorphic probes are currently being hybridized to DNA from 1,100 F. graminearum strains. Of these, 861 isolates were obtained from 7 U. S. states (47 counties) in 1999 and 2000. In addition, 239 isolates of F. graminearum originating from four wheat fields located in 3 counties in China also are being examined. Population genetic analysis of both lineage will be presented, especially in regard to gene flow and the degree of recombination within populations.