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ARS Home » Midwest Area » Madison, Wisconsin » U.S. Dairy Forage Research Center » Research » Publications at this Location » Publication #123840
Title: MARKER COMPOUNDS FOR ENZYME DEFICIENCIES IN THE LIGNIN BIOSYNTHETIC PATHWAY

Author
item LAPIERRE, CAHTERINE - INRA-GRIGNON,FRANCE
item KIM, HOON - UNIVERSITY OF WISCONSIN
item LU, FACHUANG - UNIVERSITY OF WISCONSIN
item MARITA, JANE - UNIVERSITY OF WISCONSIN
item MILA, ISABELLE - INRA-GRIGNON,FRANCE
item POLLET, BRIGITTE - INRA-GRIGNON,FRANCE
item Ralph, John

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 6/11/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Down-regulating genes for enzymes late in the lignin biosynthetic pathway can lead to the incorporation of novel monomers into lignins resulting in new structures in the polymer. For example, coniferyl and sinapyl aldehydes incorporate intimately into lignins in CAD-deficient angiosperms; new hydroxycinnamyl aldehyde 8-O-4-structures result in the lignins. Following thioacidolysis, these structures release new units not found from normal lignins which are therefore valuable marker compounds for CAD-deficiency in angiosperms. Similarly, COMT-deficient plants incorporate 5-hydroxy-coniferyl alcohol into their lignins. A 5-hydroxy-guaiacyl thioacidolysis monomer is partially released and provides a convenient marker. More diagnostic are the benzodioxane dimers released by thioacidolysis or the DFRC method.