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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #122725

Title: A SURVEY OF CALCIUM OXALATE CRYSTAL PATTERNS IN LEAVES OF THE GENUS GLYCINEAND RELATED TAXA

Author
item HORNER, HARRY - IOWA STATE UNIVERSITY
item CERVANTES M, T - IOWA STATE UNIVERSITY
item HYMOWITZ, THEODORE - UNIVERSITY OF ILLINOIS
item BROWN, ANTHONY - CSIRO PLANT INDUSTRY
item Palmer, Reid

Submitted to: Botanical Society of America Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/6/2001
Publication Date: 6/6/2001
Citation: HORNER, H.T., CERVANTES M, T., HYMOWITZ, T., BROWN, A.H., PALMER, R.G. A SURVEY OF CALCIUM OXALATE CRYSTAL PATTERNS IN LEAVES OF THE GENUS GLYCINEAND RELATED TAXA. BOTANICAL SOCIETY OF AMERICA ABSTRACTS. 2001. ABSTRACT NO. 87.

Interpretive Summary:

Technical Abstract: Calcium oxalate crystals have been identified in the majority of flowering plants, including soybean. The relationship between calcium oxalate and soluble oxalate is not known. A search for natural or induced variation in crystal numbers and patterns included mutagenized seedlings of a soybean line, transposon-tagged plants, plant introductions (accessions) of the cultivated, wild annual and wild perennial soybean, and accessions of related taxa to Glycine. Leaf samples (circular leaf punches) were taken from herbarium specimens and greenhouse and field-grown plants. The samples were chemically cleared, mounted on slides and viewed with a compound light microscope between crossed polarizers. The number and arrangement of leaf prismatic crystals (calcium oxalate, monohydrate) in an individual plant were similar but varied among accessions and species. The crystals were typically associated with the veins and lamina. The utility of leaf crystals as an aid in classification of these taxa will be presented. No accessions devoid of calcium oxalate crystals were found even though one taxon from Japan had the smallest and fewest numbers of crystals of any specimen observed.