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United States Department of Agriculture

Agricultural Research Service

Title: The Effect of Additions of Thermostable Alpha-Glucosidase on the Productionof Fermentable Sugars During Mashing

item Muslin, Elizabeth - UNIVERSITY OF WI-MADISON
item Karpelenia, Charles
item Henson, Cynthia

Submitted to: Brewers Digest
Publication Type: Abstract Only
Publication Acceptance Date: June 25, 2001
Publication Date: N/A

Technical Abstract: The thermal stability of alpha-glucosidase is important because the conversion of starch to fermentable sugars takes place at temperatures of 65-73 degrees Celsius. The thermolability of alpha-glucosidase results in either reduced efficiency of starch breakdown at the high temperatures used for starch gelatinization or requires that the starch be cooled to a more favorable temperature for enzymatic hydrolysis after the starch is gelatinized. To remedy this problem, we created a thermostable alpha- glucosidase. In this study, we tested the efficacy of the recombinant alpha-glucosidase during mashing. Mashing was conduced in the presence of non-mutated recombinant alpha-glucosidase (control) or the mutated alpha- glucosidase. The amount of glucose produced by the mashes containing the wild-type recombinant alpha-glucosidase was 9% greater than that produced by the control mashes. Experiments to determine the impact of this recombinant alpha-glucosidase upon the real degree of fermentation and the total carbohydrate profile of worts are underway.

Last Modified: 4/19/2015
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