ARS | Publication request: ISOLATION OF RAINBOW TROUT MICROSATELLITE MARKERS FROM ENRICHED LIBRARIES

Submitted to: International Plant and Animal Genome IX Conference
Publication Type: Abstract Only
Publication Acceptance Date: January 13, 2001
Publication Date: January 13, 2001
Citation: Rexroad III, C.E. 2001. Isolation of rainbow trout microsatellite markers from enriched libraries. International Plant and Animal Genome IX Conference. Jan 13-17, 2001. Abstract P613. p.214.

Technical Abstract: The global demand for seafood is expected to increase dramatically over the next two decades. Meeting this demand will require an increase in aquaculture production, since harvest yield from capture fisheries has apparently been maximized. The demand for rainbow trout is expected to follow the aquaculture trend and must be met by small-farm and industrial producers. As these producers invest more resources in aquaculture, the need arises to develop strains of fish that are genetically optimized for aquaculture. The construction of a high-density trout genetic map will allow the identification of genes affecting production traits and the selection of fish containing superior allele combinations. Two linkage maps have been published for rainbow trout. Young et al. (1998) published an AFLP linkage map containing over 470 markers. Sakamoto oet al. (2000) published a map containing over 200 markers, most of which are microsatellites. Our initial focus is to provide additional anonymous microsatellite markers for linkage mapping, contributing to the development of high-density linkage maps. Five rainbow trout microsatellite-enriched libraries were obtained from Genetic Identification Services. Clones from the libraries were sequenced and the sequences analyzed for microsatellites. PCR primer pairs were designed to flank microsatellite repeats. Primer pairs were optimized for amplification in rainbow trout and tested on other species. The efficiency of obtaining working microsatellite markers from microsatellite-enriched libraries is described in this presentation. Progress on the development