ARS | Publication request: VARIATION IN FUSARIUM GRAMINEARUM ISOLATES FROM NEPAL ASSOCIATED WITH THEIR HOST OF ORIGIN

Submitted to: National Fusarium Head Blight Forum Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: December 12, 2000
Publication Date: N/A

Technical Abstract: A collection of group II Fusarium graminearum isolates obtained from maize, wheat, and rice from different locations in Nepal was identified using a combination of morphological and molecular criteria. The variation within this collection was analyzed using RAPD markers, IGS RFLP and PCR polymorphisms. The isolates divided into two groups, designated A and B, by RAPD analysis. Isolates in group A yielded four different PCR polymorphic markers but all of the isolates in group B yielded a single polymorphic marker. The IGS RFLP analysis was consistent with the division of the isolates into the two groups. Isolates from wheat and rice were more frequently placed in group A, with isolates from maize more evenly distributed between the two groups. Results indicate that host preference might be a factor in the division of the isolates, although the year of isolation may have had an influence as well. No geographical factors or agricultural practices could be identified that could account for the observed variation.