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Title: PCR-BASED MARKERS FOR THE IDENTIFICATION OF PHOMA SCLEROTIODES INFECTING ALFALFA

Author
item Larsen, Richard
item Vandemark, George
item GRAY, F - UNIV OF WYOMING
item GRITSENKO, M - WASH STATE UNIV

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 6/18/2000
Publication Date: 7/1/2000
Citation: LARSEN, R.C., VANDEMARK, G.J., GRAY, F., GRITSENKO, M. PCR-BASED MARKERS FOR THE IDENTIFICATION OF PHOMA SCLEROTIODES INFECTING ALFALFA. CANADIAN PHYTOPATHOLOGICAL SOCIETY MEETING, JUNE 2000, p. 49. 2000.

Interpretive Summary:

Technical Abstract: Brown root rot of forage legumes is caused by the soilborne fungal organism Phoma sclerotiodes and has caused severe mortality rates in alfalfa crops grown in southwest Wyoming. Total nucleic acid extractions were made from 19 different geographical isolates of the organism. Oligonucleotide 10-mer RAPD primers have been identified from PCR reactions that reveal polymorphisms specific to P. sclerotiodes. The primers did not produce equivalent amplification products in other soil-inhabiting pathogens including Aphanomyces euteiches, Fusarium oxysporum, Verticillium albo- atrum, Phytophthora infestans, and Rhizoctonia solani. A group of at least four of the isolates originating from Boulder, WY resulted in unique polymorphisms not produced in the remaining 15 isolates when primers OpB 1, OpB 4, OpB 12 and OpC 12 were tested. This preliminary evidence suggests that the 4 isolates are phylogenetically distinct. The design of a SCAR marker specific for the detection of P. sclerotiodes is in progress.