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United States Department of Agriculture

Agricultural Research Service

Title: Mapping Maize Chromosomes Using Oat-Maize Radiation Hybrid Lines

Authors
item Okagaki, R - UNIVERSITY OF MINNESOTA
item Kynast, R - UNIVERSITY OF MINNESOTA
item Odland, W - UNIVERSITY OF MINNESOTA
item Russell, C - UNIVERSITY OF MINNESOTA
item Livingston, S - UNIVERSITY OF MINNESOTA
item Rines, Howard
item Phillips, R - UNIVERSITY OF MINNESOTA

Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: November 6, 2000
Publication Date: N/A

Technical Abstract: Radiation hybrid maps differ from conventional genetic maps by using radiation-induced breaks rather than genetic crossovers to separate markers and determine linkage relationships. Oat addition lines carrying a single maize chromosome are irradiated. The radiation treatment breaks chromosomes resulting in a random loss of sequences. The hexaploid oat background of the addition lines serves as a good host because it tolerates the loss of chromosome fragments. The maize chromosome is also fragmented, and a set of radiation hybrid lines are recovered carrying pieces of the maize chromosome either as a translocation or as maize addition chromosomes with deleted regions. Lines are screened for the presence versus absence of maize sequences, and physical linkage relationships are determined based on the probability of any two markers being present together in a particular radiation hybrid line. We are developing a chromosome 9 radiation hybrid map and producing the materials needed for mapping other maize chromosomes This material is based upon work supported by the National Science Foundation under Grant No. 9872650.

Last Modified: 11/27/2014
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