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United States Department of Agriculture

Agricultural Research Service

Title: Short-Term Cold Storage of Fly Embryos: Effects on Survival and Fitness Factors

Author
item Leopold, Roger

Submitted to: Society for Cryobiology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 31, 2000
Publication Date: N/A

Technical Abstract: During mass-rearing of insects for control programs, it is beneficial to be able to hold eggs needed for propagation for short periods of time ranging from several hours to several days. In this study, the survival of house fly, Musca domestica, embryos was evaluated for up to 8 days following storage at 5 deg. C. Tolerance to chilling was influenced by the length of the storage period and by the age of the embryos when placed into storage. Embryos placed into the cold at 3 hr after oviposition were able to survive 3 days without significant reduction in hatching or development to the adult stage. One-hr-old embryos were the least tolerant to cold and survive less than 1 day at 5 deg. C. Three patterns of chilling injury expression were revealed by this study. The patterns were characterized as immediate, accumulative, and latent. The expression of immediate and accumulative chilling injury was linked to the age of the embryos when they were placed into storage and both types of injury expression were manifested during embryogenesis. The latent expression of injury incurred during embryonic cold storage was detected during the post-embryonic stages and was related to the length of exposure to the cold. Latent chilling injury was frequently displayed as eclosion failure and lack of vitality during the adult stage. Exposure of the house fly embryos to hypoxic conditions had no noticeable effect on chilling tolerance. A hyperoxic environment during cold storage reduced survival.

Last Modified: 9/22/2014
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