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United States Department of Agriculture

Agricultural Research Service

Title: The Use of An Antigen Based on Molecular Modeling Resulted in the Development of a Monoclonal Antibody-Based Immunoassay for the Coccidiostatnicarbazin

Authors
item BEIER, ROSS
item STANKER, LARRY

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 15, 1999
Publication Date: N/A

Technical Abstract: Nicarbazin is a feed additive used to prevent outbreaks of cecal and intestinal coccidiosis in poultry, and is composed of equimolar amounts of 4,4'-dinitrocarbanilide (DNC) and 2-hydroxy-4,6-dimethylpyrimidine (HDP). The drug also is used to increase the rate of weight gain in poultry. DNC is the active component in nicarbazin and analysis of DNC is used to determine nicarbazin residues in tissue. Currently, detection of tissue residues and feed levels are limited to laboratory-based liquid chromatographic techniques. It would be advantageous to produce antibodies to DNC and develop an ELISA that could be used in screening tests for DNC. Antibodies to DNC have not been previously produced. Antibodies to DNC have now been produced with the use of a DNC mimic immunogen proposed from molecular modeling studies. Due to the low solubility of DNC in aqueous systems, a solvent system was developed that both provided appropriate solubility of DNC and that could be used in an ELISA. Finally, the use of an appropriate plate-coating antigen resulted in the successful development of monoclonal antibodies to nicarbazin.

Last Modified: 9/10/2014
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