|Sengupta-Gopalan, Champa - NEW MEXICO STATE UNIV.|
Submitted to: Plant Physiology Plant Gene Register Electronic Submission
Publication Type: Abstract Only
Publication Acceptance Date: September 16, 1998
Publication Date: N/A
Technical Abstract: Glutamine Synthetase (GS) is a key enzyme in the assimilation of ammonia . GS catalyzes the ATP-dependent condensation of ammonia with glutamate to form glutamine. GS isoforms are either located in the cytosol (GS1) or the chloroplasts (GS2) . In legumes, GS1 is encoded by members of a differentially regulated small multigene family. Legume nodules receive a large influx of ammonia due to the nitrogen reducing activity of symbiotic bacteria belonging to the species Rhizobium and Bradyrhizobium In soybean nodules, both constitutively expressed and nodule enhanced members of the GS1 gene family have been described that are upregulated in nodules and probably assimilate symbiotically produced ammonia. We have previously characterized a 3* partial cDNA clone (pGSGmE GenBank no L20248) that appears to be expressed in a nodule-specific manner and we report here the full length genomic sequence corresponding to this gene.